Table 3.
Baseline | H₂O₂ | ||||||||
---|---|---|---|---|---|---|---|---|---|
PCL | LV base | LV apex | LV base | LV apex | |||||
(ms) | APD90 | CaTD90 | APD90 | CaTD90 | APD90 | CaTD90 | APD90 | CaTD90 | |
300 | NR | 112 ± 2 | 195 ± 4 | 115 ± 6 | 190 ± 3 | 113 ± 3 | 195 ± 16 | 116 ± 1 | 185 ± 15 |
SHR | 128 ± 7***, ††† | 178 ± 21 | 108 ± 4† | 170 ± 8††† | 135 ± 14**, †† | 200 ± 2* | 107 ± 7 | 185 ± 3 | |
250 | NR | 98 ± 9 | 167 ± 7 | 96 ± 5 | 164 ± 8 | 100 ± 4 | 143 ± 4 | 99 ± 12 | 136 ± 12 |
SHR | 114 ± 19 | 142 ± 7††† | 99 ± 18 | 141 ± 9†, † | 125 ± 11*, ††† | 149 ± 4† | 104 ± 19 | 149 ± 20 | |
200 | NR | 94 ± 8 | 123 ± 3 | 93 ± 17 | 125 ± 2 | 94 ± 9 | 125 ± 12 | 92 ± 10 | 124 ± 18 |
SHR | 110 ± 9† | 120 ± 3 | 99 ± 15 | 121 ± 7 | 119 ± 14*, †† | 130 ± 7 | 102 ± 18 | 124 ± 7 | |
150 | NR | 88 ± 16 | 112 ± 9 | 87 ± 14 | 114 ± 1 | 91 ± 6 | 115 ± 7 | 88 ± 10 | 114 ± 2 |
SHR | 100 ± 8 | 116 ± 6 | 88 ± 15 | 115 ± 6 | 115 ± 7*, ††† | 124 ± 6† | 89 ± 17 | 120 ± 11 |
Simultaneous voltage and calcium imaging (four pairs of recordings at the LV base and four pairs at the LV apex per heart, per PCL) were performed for six NR and six SHR hearts before and after perfusion with H2O2. Data are reported as the mean ± SD. Intragroup comparison: *P < 0.05, **P < 0.005, ***P < 0.0005; intergroup comparison: † P < 0.05, †† P < 0.005, ††† P < 0.0005; the bootstrap method.