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. 2015 Dec 20;594(6):1643–1661. doi: 10.1113/JP271309

Figure 3. Acute hypercapnia reduces adenosine but not IBMX‐stimulated transepithelial anion secretion in Calu‐3 cells .

Figure 3

Calu‐3 cells were grown on permeable Snapwell supports and I sc was measured using an Ussing chamber. For control experiments, cells were gassed with 5% (v/v) CO2/95% (v/v) O2 whilst hypercapnia was induced by pre‐exposing cells to 10% (v/v) CO2/90% (v/v) O2 for 20 min prior to being studied. Apical [Cl−] was reduced to 40 mm and cells were stimulated with either adenosine (10 μm) or IBMX (1 mm) before addition of apical CFTRinh‐172 (20 μm) and basolateral bumetanide (25 μm). A, the maximal adenosine‐stimulated increase in I sc; B, the rate of increase in adenosine‐stimulated I sc. *Significant effect of hypercapnia (P < 0.05). Data represent mean ± SEM; n = 5 for normocapnia and n = 3 for hypercapnia. C, the maximal IBMX‐stimulated increase in I sc; D, the rate of increase in IBMX‐stimulated I sc. Data represent mean ± SEM; n = 3 for normocapnia and n = 4 for hypercapnia.