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. Author manuscript; available in PMC: 2016 Mar 20.
Published in final edited form as: Radiat Res. 2015 May 14;183(6):594–609. doi: 10.1667/RR14016.1

Figure 8. Loss of HIF-1α was correlated with decreased mitochondrial biogenesis in sarcoma cells in response to radiation.

Figure 8

Primary cells from P7NP and P7NPH1 tumors were isolated as described in Figure 3. All experiments were performed with in vitro cultures under 21% oxygen. A) The mitochondrial density in P7NP and P7NPH1 cells in response to 6Gy irradiation was measured first by incubating nonirradiated and irradiated cells with the MitoTracker DeepRed reagent after 72 hours, then by performing flow cytometry. Signal from irradiated cells were normalized to nonirradiated controls. The P7NPH1 cells had significantly decreased mitochondrial density at 72 hours following irradiation when compared to P7NP cells by a two-tailed student t-test. B) The mitochondrial DNA (mtDNA) content was measured via real-time PCR using DNA extracted from purified P7NP and P7NPH1 sarcoma cells at 72 hours after 6Gy irradiation, and normalized to 18S and nonirradiated controls. P7NP cells had more mtDNA content at 72 hours following irradiation as compared to P7NPH1 cells by a two-tailed student t-test. * p<0.05. ** p<0.01.