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Coimmunoprecipitation of CSRP1 with Three Subunits of the ASC-1 Complex
Immunoprecipitation was done with anti-TRIP4, anti-ASCC1, and anti-ASCC2 antibodies. For mock immunoprecipitation, we used GAPDH antibodies, and for empty controls, we used unloaded Protein-G-Sepharose beads. Western blots from input, flow-through (unbound), and bound protein (elution) were serially incubated with anti-TRIP4, anti-ASCC1, anti-ASCC2, as well as with anti-CSRP1 antibodies.
