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. 2016 Mar 3;9(1):25–31. doi: 10.1016/j.tranon.2015.11.005

Figure 3.

Figure 3

MiR-200a directly and negatively regulated DEK gene. (A) miR-200a was significantly upregulated in normal pancreatic tissues compared with pancreatic tissues. miR-200a was detected using qRT-PCR in 81 cases of fresh pancreatic cancer tissues and its paired normal control tissues. (B) Basal level of miR-200a was detected using qRT-PCR technique in four different pancreatic cancer cell lines (PK-1, KLM-1, PK-8, and AsPC-1). (C) Endogenous expression level of DEK gene was detected using Western blot in four different pancreatic cancer cell lines (PK-1, KLM-1, PK-8, and AsPC-1). (D) DEK gene was bioinformatically predicted as target of miR-200a using online software (http://www.microrna.org/). (E) Luciferase reporter assay was performed to confirm that DEK gene was a direct and downstream target of miR-200a. (F) Western blot was carried out finding that miR-200a negatively regulated DEK gene. Independent-sample t test was carried out to analyze the statistical difference. *P < .05, **P < .01, and ***P < .001 compared with control group.