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. 2016 Mar 3;9(1):25–31. doi: 10.1016/j.tranon.2015.11.005

Figure 4.

Figure 4

MiR-200a can significantly suppress the proliferation, migration, and invasion of pancreatic cancer cell lines. (A) MTT assay was performed to analyze the proliferative variation after transfection with miR-200a mimics into AsPC-1 cells where basal level of miR-200a was lowest among the four different kinds of pancreatic cancer cell lines and transfection with miR-200a-inhibitor into PK-1 cells where basal level of miR-200a was highest among the four different kinds of pancreatic cancer cell lines. The absorbance value was detected at 24, 48, 72, and 96 hours posttransfection. (B) Wound-healing assay was qualitatively and quantitatively carried out to detect the migratory variation in PK-1 and AsPC-1 cells after transfection with miR-200a inhibitor and miR-200a mimics, respectively. (C) Transwell assay was conducted to analyze the invasive variation in PK-1 and AsPC-1 cells after transfection with miR-200a inhibitor and miR-200a mimics, respectively. Independent-sample t test was employed. *P < .05 and **P < .01 in comparison with control group.