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. 2016 Mar 3;9(1):70–78. doi: 10.1016/j.tranon.2016.01.004

Figure 3.

Figure 3

Droxinostat induces HepG2 cell apoptosis by activating the mitochondrial apoptotic pathway and downregulating expression of FLIP. (A) Apoptotic cells were determined via flow cytometry following staining with Annexin V and PI. (B) Apoptosis rates of HepG2 cells treated with different concentrations of droxinostat were analyzed using bar graphs. (C) mRNA levels of genes related to the mitochondrial p53 apoptosis pathway were analyzed using qRT-PCR. (D) Levels of proteins associated with the mitochondrial p53 apoptosis pathway were analyzed via Western blot. (E) The relative contents of proteins of the mitochondrial p53 apoptosis pathway were analyzed using bar graphs. (F) Bax/Bcl-2 protein ratio. (G) Protein levels related to FLIP and caspase-8 were analyzed via Western blot. (H) Relative FLIP and caspase-8 levels were analyzed using bar graphs. Data are shown as means ± SE of three independent experiments, *P < .05, **P < .01, compared with untreated control cells (no droxinostat).