Figure 3.

Psammomys obesus aortic smooth muscle cells (VSMCs), exposed at 0.6% of D-Glucose for 48 hours and treated with aqueous extract of Brassica rapa var. rapifera at 2100 μg/mL for 24 hours. (a) Control, (b) control treated with AEBr, (c, d, e) VSMCs group exposure at high dose of D-Glucose, and (f) VSMCs exposure to glucotoxicity and treated with AEBr. The cells were fixed in aqueous Bouin and stained with May Grunwald-Giemsa (MGG); the observation was done with an inverted microscope (Zeiss) (GX400). Blue arrow: cytosolic vacuolization = oncosis, yellow arrow: fragmented nuclei fragmentation, and green arrow: apoptotic crop.