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Histological analysis. To verify the sites of microinjections, methylene blue was injected into three rats, and the brains were sliced as 30 μm coronal sections. Methylene blue was detected at a 3 mm radial spread from the injection site. (Left) diagram of coronal section at -3.12 mm from bregma; ○ cannulation site, ○ injection site. (Right) photomicrographs showing cannula placement in the three rats.
