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Agar disk diffusion assay. (A) Paper disks impregnated with control DMSO or 3.125, 6.25, 12.5, 25, 50, and 100 μg of triclosan were placed on the agar plate containing Cryptococcus neoformans. Diameters of the inhibition zone were measured after 48 h of incubation. Data shown is a representative picture of three independent experiments. (B) Graph shows the inhibition diameter (mm) of C. neoformans plate after triclosan treatment. Data shown are mean ± SD. ∗P < 0.05, ∗∗P < 0.01.
