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. 2016 Mar 21;7:360. doi: 10.3389/fmicb.2016.00360

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Copyright © 2016 Movahed, Tan, Munusamy, Yeow, Tay, Wong and Looi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cell morphology of triclosan-treated C. neoformans. Electron microscopic pictures of C. neoformans with or without exposure to triclosan (0.5 μg/ml) for 2 h. (A,B) Untreated control cells showed intact nucleus and cytoplasm. (C–F) Triclosan-treated C. neoformans showed apoptotic morphologies. Note the appearance of apoptotic features such as mitochondrial swelling (arrow) and nuclear chromatin condensation (broken arrow). Besides, intense cytoplasmic vacuolations were formed in the cells. Cell surface fibrilar structures which form capsule polysaccharide component were also disrupted (Pavel and Vasile, 2012).