FIG. 2.

Particle staining within polyHIPEs and films illustrates the successful incorporation and relative location of the respective particles. Alizarin red was used to stain for calcium ions and picrosirius red used to stain collagen. (A) Negative staining of alizarin red of HA nanoparticles in propylene fumarate dimethacrylate (PFDMA) film indicates minimal surface enrichment. (B) Alizarin red staining of polyHIPEs cultured in growth media through 2 weeks. All polyHIPEs stained positive for calcium after two weeks, indicating the deposition of calcium from the media. (C) Picrosirius red staining illustrating the collagen-rich DBM particles in the polyHIPE. Color images available online at www.liebertpub.com/tea