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. 2016 Mar 2;22(5-6):436–448. doi: 10.1089/ten.tea.2015.0284

FIG. 3.

FIG. 3.

ELISA quantification for collagen type I and gene expression levels of human vascular and avascular meniscus cells cultivated on either random or aligned collagen ES scaffolds. (A) ELISA quantification of collagen type I for cell- and noncell-seeded aligned collagen and PLA scaffolds. (B) COL1A1 gene expression. (*p = 0.0026 among experimental groups, Kruskal–Wallis test). (C) SOX9 gene expression. (D) COMP gene expression. (E) CHAD gene expression. (F) Aggrecan gene expression (#p = 0.0441 among experimental groups, Kruskal–Wallis test) (n = 4–6 donors) relative to monolayer controls. Expression levels are relative to monolayer controls (red dotted line). Cells that were derived from either vascular or avascular regions cultivated on both random and aligned collagen scaffolds expressed significantly (p < 0.05, Kruskal–Wallis test) higher levels of COL1A1, SOX9, COMP, CHAD, and AGG gene expression relative to monolayer cultured cells. ELISA, enzyme-linked immunosorbent assay.