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. 2015 Jun 22;35(11):1373–1385. doi: 10.1038/onc.2015.193

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3-ketosteroids induce a CK5+ cell population in ERα-positive breast cancer cell lines. Flow cytometry of CK5+ cells following exposure to 3-ketosteroids or estrogen for 6 days in T47D (a) and MCF7 (b) cell lines. Representative frequency plots of cellular CK5-fluorescence by flow cytometry in T47D cells treated with dexamethasone (Dex; c) or progestin (Prg; d) compared with vehicle (Veh). Representative images of fluorescence-based immunocytochemistry of T47D (e) and MCF7 (f) cell cultures following exposure to 3-ketosteroids for 6 days and stained for CK5 (red) and DAPI (blue).