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3-ketosteroid-treated T47D cells grow in soft agar and express stem cell marker CD44. Representative images of T47D cell colonies in soft agar following stimulation with individual 3-ketosteroids for 6 days (a). Scatter dot plot of colony number per field (b) and colony size per field (c). Mean and s.e.m. are indicated. Multilaser flow cytometry showing CK5 and CD44 expression in T47D cells stimulated with 3-ketosteroids or β-estradiol for 6 days (d).
