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. 2016 Mar 21;6:23375. doi: 10.1038/srep23375

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After being treated by antibiotics for 0, 0.5, 1 and 2 hours, the bacteria were stained with PI (fluorescing red) and SYTO 9 (fluorescing green). BF: bright-field image; FL: fluorescence image. (a) S. aureus (ATCC 29213) treated with oxacillin; (b) E. coli (ATCC 25922) treated with imipenem; (c) percentage of dead bacteria (f_dead): S. aureus (black columns) and E. coli (gray columns). f_dead = N_r/(N_g + N_r − N_g+r), where N_r is the count of the red signatures, N_g is the count of the green signatures, and N_g+r is the count that the green and red signatures overlap. The scale bars represent 20 μm.