Table 2. Calibration data for each standard ecdysteroid.

Compound	Regression equation	R2	Linear range (ng/mL)	LODa (ng/mL)	LOQb (ng/mL)
20E	y = 59.27x + 44.0	0.9988	1.40–1428.6	0.24	0.79
pB	y = 36.61x + 19.1	0.9998	1.40–1428.6	0.20	0.66
pS	y = 78.44x + 28.3	0.9998	1.40–1428.6	0.34	1.12
E	y = 67.86x + 22.0	0.9995	5.58–1428.6	0.77	2.56
2d20E	y = 98.14x + 66.2	0.9996	1.40–1428.6	0.27	0.89
ajC	y = 32.42x–47.7	0.9999	1.40–1428.6	0.38	1.27
Ds	y = 134.57x + 26.8	0.9995	0.35–1428.6	0.14	0.47

Abbreviations: 20-hydroxyecdysone (20E), polypodine B (pB), poststerone (pS), ecdysone (E), 2-deoxy-20-hydroxyecdysone (2d20E), ajugasterone C (ajC), dacryhainansterone (Ds). ^aLOD: limit of detection.

^bLOQ: limit of quantification.