Table 3. Optimized LC-MS/MS conditions for each standard ecdysteroid.
| Compound | Retention time (min) | Quantitative MRM transition | CE (eV) | Qualitative MRM transition | CE (eV) | Fragmentor voltage (V) |
|---|---|---|---|---|---|---|
| 20E | 2.43 | 481 > 445 | 16 | 481 > 165 | 24 | 135 |
| pB | 2.53 | 497 > 443 | 20 | 497 > 369 | 24 | 135 |
| pS | 3.7 | 363 > 345 | 12 | 363 > 215 | 22 | 100 |
| E | 4.82 | 447 > 429 | 20 | 447 > 109 | 28 | 135 |
| 2d20E | 5.52 | 465 > 429 | 16 | 465 > 355 | 20 | 135 |
| ajC | 5.65 | 481 > 427 | 16 | 481 > 299 | 22 | 135 |
| Ds | 6.77 | 463 > 299 | 20 | 463 > 209 | 26 | 135 |
Abbreviations of compounds can be found in the legend of Table 2.