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. 2016 Mar 17;7:10932. doi: 10.1038/ncomms10932

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The complex was produced in (a) E. coli, (b) insect and (c) mammalian cells. Proteins were produced and purified separately. The complex was formed by mixing the two proteins and by the removal of the solubilizing agents followed by a gel filtration chromatography. The elute fractions were analysed by SDS–PAGE.