Table 1. Proteins complexes produced using the strategy described.

	Protein	Stabilized by	Strategy	References
Human steroid nuclear receptors	ERα-E	Ligand (oestrogen)	Ligand in cell culture	14
		Thioredoxin fusion	Keep Thioredoxin (TRX) fusion for crystallization	33
		3Cys to Ser mutations	Mutations mimic the conformational changes induced by the ligand	13
	ERβ-EF	TIF2 (623–772)	In vitro reconstitution (co-cell lysis)	This article
	GRtm-E	TIF2 (623–772) and solubility mutant	In cell co-expression	This article
HIV-1 pre-integration complexes	IN	hLEDGF	In vitro reconstitution (dialysis)	12
		hLEDGF/hINI1(174–289)		11
		hLEDGF	Expression in E. coli, insect and mammalian cells and in vitro reconstitution	This article

ER, human estradiol (E2) nuclear receptor; IN, integrase; TIF2, transcriptional intermediary factor 2.