Table 2. Comparison of the failure reason of pyrosequencing between BamHI treated group and non-BamHI treated group.

Failure reason	Non open-loop group				Open-loop group
	MT1	MT3	MT10	MT13	MT1	MT3	MT10	MT13
Blood
Failed bisulfite conversion	100%(16/16)	10%(1/10)	81.82%(27/33)	89.19%(33/37)	0	50%(2/4)	0	6.67%(1/15)
Lack of data	0	10%(1/10)	0	0	28%(7/25)	0	33.33%(1/3)	13.33%(2/15)
Possible dispensation error/Failed reference sequence pattern	0	0	0	2.70%(1/37)	72%(18/25)	0	0	0
Low signal-to-noise ratio	0	80%(8/10)	18.18%(6/33)	8.11%(3/37)	0	50%(2/4)	66.67%(2/3)	80%(12/15)
Saliva
Failed bisulfite conversion	0	0	0	0	0	66.67%(2/3)	0	0
Lack of data	50%(1/2)	0	0	0	100(3/3)	0	0	0
Possible dispensation error/Failed reference sequence pattern	0	0	0	0	0	33.33%(1/3)	0	0
Low signal-to-noise ratio	50%(1/2)	100%(2/2)	100%(2/2)	100%(1/1)	0	0	100%(1/1)	100%(2/2)