Abstract
A simple, rapid, and sensitive method for the determination of iron in serum or plasma is described.
The procedure is carried out at room temperature with 2 ml. of serum or plasma, or with 1 ml. if high values are expected; it can be applied to turbid or jaundiced samples, whether previously frozen or not.
An ethanolic solution of 4: 7-diphenyl-1: 10-phenanthroline is used to produce a coloured iron complex, the optical density of which can be measured in any suitable photometer, using either 10 or 20 mm. fused glass cuvettes or matched tubes of 1.1 cm. internal diameter.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BOTHWELL T. H., MALLETT B. The determination of iron in plasma or serum. Biochem J. 1955 Apr;59(4):599–602. doi: 10.1042/bj0590599. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fowweather F. S. The determination of iron in blood-plasma. Biochem J. 1934;28(4):1160–1164. doi: 10.1042/bj0281160. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LAURELL C. B. Plasma iron and the transport of iron in the organism. Pharmacol Rev. 1952 Dec;4(4):371–395. [PubMed] [Google Scholar]
- PETERS T., GIOVANNIELLO T. J., APT L., ROSS J. F. A simple improved method for the determination of serum iron. II. J Lab Clin Med. 1956 Aug;48(2):280–288. [PubMed] [Google Scholar]
- RAMSAY W. N. M. The determination of iron in blood plasma or serum. Biochem J. 1953 Jan;53(2):227–231. doi: 10.1042/bj0530227. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SEVERINGHAUS J. W., FERREBEE J. W. Calcium determination by flame photometry; methods for serum, urine, and other fluids. J Biol Chem. 1950 Dec;187(2):621–630. [PubMed] [Google Scholar]
- TRINDER P. The improved determination of iron in serum. J Clin Pathol. 1956 May;9(2):170–172. doi: 10.1136/jcp.9.2.170. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tompsett S. L. Thiolacetic acid as a reagent for the determination of the inorganic iron content of certain biological materials. Biochem J. 1934;28(4):1536–1543. doi: 10.1042/bj0281536. [DOI] [PMC free article] [PubMed] [Google Scholar]