Skip to main content
. 2015 Sep;100(9):1160–1171. doi: 10.3324/haematol.2014.120295

Figure 7.

Figure 7.

In combination with SDF-1α treatment, severe hypoxia improves leukemic cell survival of AML cell lines and AML-M5 cells treated with Ara-C. (A) SDF-1α significantly increases cell survival of Ara-C-treated U937 cells (+SDF-1α) compared to untreated cells, in normoxia and this effect is enhanced in hypoxia. (B) Flow cytometry analysis shows CXCR4 protein overexpression in HL-60(CXCR4) cells as compared to HL-60(Empty) control cells. (C) SDF-1α induces a significant increase in Ara-C-treated HL-60(CXCR4) cell survival (+SDF-1α) compared to untreated cells, in normoxia and this effect is enhanced in hypoxia. (D) Induction of apoptosis decreases in Ara-C-treated HL-60(CXCR4) cells grown on a layer of MS5 stromal cells (MS5), compared to standard culture (control, C). (E, F) In primary leukemic blast cells obtained from two patients with AML-M5, SDF-1α increases cell survival of Ara-C-treated AML-M5 (13) and (14) cells (+SDF-1α), compared to untreated cells, in both normoxia and hypoxia. (A, C, D) Mean ± SEM values from three independent experiments are shown. *P<0.05, **P<0.01. (B, E, F) One representative experiment is shown.