Table 2. Kinetic parameters of polyol dehydrogenases.
Mannitol |
Sorbitol |
|||
---|---|---|---|---|
vmax1 | KM1 | vmax1 | KM1 | |
YNR073C | 0.40 ± 0.02 | 3.6 ± 0.5 | 2.1 ± 0.5 | 634.9 ± 207.1 |
Dsf1 | 0.90 ± 0.02 | 2.9 ± 0.3 | 1.5 ± 0.7 | 869.6 ± 601.8 |
Sor1 | 0.10 ± 0.02 | 89.0 ± 37.5 | 0.40 ± 0.01 | 11.8 ± 1.4 |
Sor2 | 0.05 ± 0.01 | 98.4 ± 25.7 | 0.5 ± 0.02 | 15.1 ± 2.8 |
vmax values are given as μmol substrate converted per minute per mg of total protein; KM values are in mM.
1calculated from data presented in Supplementary Fig. S6.