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. Author manuscript; available in PMC: 2016 Mar 21.
Published in final edited form as: Bioconjug Chem. 2006 May-Jun;17(3):823–830. doi: 10.1021/bc060006z

FIGURE 3.

FIGURE 3

Relative type α1(I) collagen gene transcription in HSC-T6 cells treated with TFO or (M6P)20-BSA-TFO by real time RT-PCR assay. HSC-T6 cells were treated with unconjugated TFO (0.5 μM) or (M6P)20-BSA-TFO at indicated concentrations in 1 ml of FBS free DMEM for 24h and then 1 ml of DMEM containing 20% of FBS was added and incubated for another 24h. Relative transcript levels were normalized using 18S rRNA as a housekeeping gene. Control means no treatment. Values are mean ± SD of 3 independent experiments. Asterisk: statistically significant compared to control group; n.s.: not significant.