FIG 7.

Mapping of the Pbs2 binding domain in Ahk1. (A, C, and E) Schematic models of the Ahk1 deletion constructs used in the experiments shown in panels B, D, and F, respectively. (B, D, and F) In vivo assays of the coIP of Ahk1 and Pbs2. TM257 was cotransformed with the expression plasmid for the HA-tagged N-terminal fragment of Pbs2 [HA-Pbs2(1-240)] and the mutant derivatives of GST-Ahk1 depicted in panels A, C, and E, all under the control of the GAL1 promoter. CoIP assays were conducted essentially as described for Fig. 6B.