FIG 8.

Mapping of the Ste11 binding domain in Ahk1. (A, B, and D) In vivo assays of the coIP of Ahk1 and Ste11. TM257 was cotransformed with an expression plasmid for HA-tagged full-length Ste11 (HA-Ste11-FL) and the mutant derivatives of GST-Ahk1 depicted in Fig. 7A and C and in panel C, all under the control of the GAL1 promoter. CoIP assays were conducted essentially as described for Fig. 6B. In panels B and D, NaCl was not added. vec, GST vector. (C) Schematic models of the Ahk1 deletion constructs used in the coIP assays shown in panel D. (E) Schematic summary of the results of the mapping of binding domains in Ahk1 (described in the legends for Fig. 5 and 7 and for this figure).