FIG 10.

RNAi does not contribute to silencing in T. delbrueckii. (A) Expression of HMRa1 in the wild type, ago1Δ and dcr1Δ mutants, an ago1Δ dcr1Δ double mutant, and a sir2Δ mutant. Repression of a1 was maintained in all three RNAi mutants. The error bars indicate standard deviations. (B) Heat map displaying significant changes in expression of genes across the three RNAi mutants, as well as the 22 genes that increased in expression across all three sir mutants. All expression changes were filtered for genes that increased or decreased in expression more than 2-fold relative to the wild type and showed a false-discovery rate of <10%. For genes with orthologs in S. cerevisiae, the three-letter gene name is shown. Whole-genome duplicates are labeled with the names of both S. cerevisiae duplicates (e.g., “RGI1/RGI2” represents the pre-whole-genome-duplication ancestor of these two genes in T. delbrueckii). (C and D) Weighted Venn diagrams of overlapping genes increasing and decreasing, respectively, in expression relative to the wild type in each of the RNA mutants and the double mutant.