FIG 1.

Effects of knocking down FAF1 on the immune signaling pathway. HeLa cells were transfected with a control or FAF1 siRNA 1. After 48 h, cells were harvested and examined. (A and B) Differentially expressed genes were analyzed using a microarray. Genes (fold change of ≥1.5) were analyzed using Ingenuity Pathway Analysis (IPA), and predicted signaling pathways are listed with their P values (A). Heat maps (B) show microarray analysis results. Genes up- or downregulated more than 2-fold in FAF1-knockdown cells and some representative genes are listed (left side of the heat map) with their log₂ ratios (right side of the heat map). Columns s1, s2, and s3 indicate biological triplicates of the experiment. (C) mRNA levels of IFIT1, IFIH1, and IFN-β were measured using RT-qPCR. The values were normalized to GAPDH mRNA values and represent the means ± standard deviations of three experiments. (D) Protein levels of FAF1, Mx1, STAT1, ISG15, and RIG-I were analyzed using Western blot analysis. Actin bands are shown as loading controls. s1 and s2 indicate biological duplicates of the experiment. IB, immunoblot.