FIG 9.

FAF1 does not affect nuclear export of IRF3 and recruitment of CBP to IRF3. (A) HeLa cells were cotransfected with IFN-β-Luc, beta-Gal, Flag-FAF1, or a control plasmid. After 24 h, cells were treated with poly(I·C) (10 μg/ml) and incubated in the presence or absence of LMB (20 nM). After 6 h of incubation, cells were harvested, and relative luciferase activities were measured. The data represent the means ± standard deviations of three experiments. ***, P < 0.001 (for differences between values for Flag and Flag-FAF1). (B) HeLa cells were transfected with a control siRNA or FAF1 siRNA 2 for 48 h and then transfected with enhanced GFP, IRF3-WT-GFP, or IRF3-5D-GFP. At 24 h posttransfection, cell lysates were immunoprecipitated with anti-GFP antibody, and immune complexes were analyzed by Western blotting.