FIG 4.

PLD-targeting microRNA expression is increased in breast cancer cells in response to long-term starvation. (A to H) Cells were deprived of serum for the indicated lengths of time. qRT-PCR was performed to measure endogenous levels of miRNAs, i.e., miR-182 (A, B), miR-203 (C, D), miR-887 (E, F), and miR-3619-5p (G, H) in MCF-7 cells and MDA-MB-231 cells, respectively. (I) Endogenous gene expression of PLD-targeting miRNAs under nonstarved and starved (24 h) conditions in noncancer cells, MCF-10. Error bars are for results from three independent experiments performed in duplicate; n = 5 for measurement of miR-3619-5p at 24 h of starvation. * and #, statistically significant (P < 0.05) ANOVA increases or decreases, respectively, between samples and controls.