Figure 2. Reactive oxygen species are increased in HMVEC when SAEC are treated with PEPs.
HMVEC were treated with 5 µM DHE for the last 30 min of 24 h treatment of SAEC with (A) control, (B) PEPs PM0.1 0.5 µg/mL, (C) PEPs PM0.1 1.0 µg/mL, (D) PEPs PM2.5 1.0 µg/mL, (E) SiO2 1.0 µg/mL, and (F) WF 1.0 µg/mL. (G-L) HMVEC were pre-treated with 2000 U/mL catalase for 1 h. Images were taken using a Zeiss LSM510 microscope. Images are a representation of n = 3.