A. The experimental timeline is shown. Six weeks after sham-treatment or X-irradiation, electrodes were implanted (surgery). One week later, mice were pretreated with Etx and injected with KA after 30 min. Sample sizes are as follows: 20 mg/kg, n = 8/group; 25 mg/kg, n = 3/group.
B. 1–2. Representative traces show that convulsive seizures (arrows) occurred sooner and were greater in number in X-irradiated mice compared to sham controls.
C. Dividing the 3 hrs after KA into 15 min-long bins shows that X-irradiated mice had more convulsive seizures (stages 3–5, Racine, 1972). Each row is for a different mouse. Right: Colors are used to indicate the number of convulsive seizures/bin, ranging from 0 (blue) to 8 (red).
D. 1–2. X-irradiated mice that were administered 20 mg/kg KA had a shorter latency to the first convulsive seizure, but not the first nonconvulsive seizure. 3. X-irradiated mice had no significant differences from Sham-irradiated mice in the numbers of seizures. 4. Like GFAP-TK+ mice (Fig. 1D4), there was a longer total convulsive seizure duration and shorter nonconvulsive seizure duration in X-irradiated mice compared to Sham-irradiated mice.