Table 1.

Primer sequence and reaction condition of miR-206 and Cx43 by qRT-PCR

Primer	Sequence (5’-3’)	Reaction condition of qRT-PCR
Cx43	F: GGTGGAGAGGGAGGGGATAAGAG R: CCACCCGCTCATTCACATACAC	95°C 1: 30→(95°C, 5 s→62.5°C, 30 s) × 40→65°C, 1 s
GAPDH	F: AGAGGCAGGGATGATGTTCTG R: GACTCATGACCACAGTCCATGC	95°C 1: 30→(95°C 5 s→62.5°C 30 s) × 40→65°C 1 s
miR-206 Reverse-transcribed primer:  GTCGTATCCAGTGCAGGGTCCG AGGTATTCGCACTGGATACGACCCACACA	F: GGCCGCTGGAATGTAAGGAAG R: GTGCAGGGTCCGAGGT	95°C 1: 30→(95°C, 5 s→60°C, 30 s) × 40→65°C, 1 s
U6 Reverse-transcribed primer:  AAAATATGGA ACGCTTCACGAATTTG	F: CTCGCTTCGGCAGCACATATACT R: GTAACGCTTCACGAATTTGCGTGTC	95°C 1: 30 →(95°C 5 s→60°C 30 s) × 40→65°C 1 s

Cx43: Connexin 43; qRT-PCR: Quantitative real-time polymerase chain reaction; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; miR-206: MicroRNA-206.