FIG 3.
V. cholerae FeoB makes complexes in vivo that are located in the inner membrane. (A) A culture of EPV6/pFeoAB-V5C was cross-linked using formaldehyde (CH2O), and the proteins were separated by SDS-PAGE and analyzed by immunoblotting using an anti-V5 antibody. (B) The lysate from cross-linked cells was fractionated to separate the cytoplasm (Cyto), inner membrane (IM), and outer membrane (OM). Proteins were resolved by SDS-PAGE and analyzed by immunoblotting using an anti-V5 antibody. The monomeric form of FeoB is shown with an asterisk.