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. 2016 Mar 17;198(7):1160–1170. doi: 10.1128/JB.00930-15

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FIG 5 — Immunoprecipitation of in vivo cross-linked Feo complexes using V5-tagged FeoB or FeoC. (A) Proteins were formaldehyde cross-linked in vivo and immunoprecipitated using a mouse anti-V5 antibody. Cultures of EPV6 containing the empty vector (pWKS30), a plasmid encoding the Feo proteins with V5-tagged FeoB (pFeoAB^-V5C), or a plasmid encoding the Feo proteins with V5-tagged FeoC (pFeoABC^-V5) were grown to mid-log phase and cross-linked with formaldehyde. Proteins were separated by SDS-PAGE and visualized using Gel Code Blue. Numbered arrowheads indicate Feo complexes that were excised and analyzed by mass spectrometry. The asterisk denotes the monomeric FeoB. (B and C) V5-labeled complexes immunoprecipitated from either cross-linked FeoB-V5 (B) or cross-linked FeoC-V5 (C) were visualized with a rabbit-derived anti-V5 antibody.