TABLE 2.
Primers used in this study
| Primer | Sequence (5′–3′) |
|---|---|
| pFeo101.FeoB.Nsi.Fwd | TGTGGAGAGAGCACAATGATGCATCAAGTACTCACCGTAGG |
| pFeo101.FeoB.Nsi.Rev | CCTACGGTGAGTACTTGATGCATCATTGTGCTCTCTCCACA |
| FeoB.V5.Fwd | TGGCAAGCCCATCCCCAACCCCTTGCTTGGCTTGGACTCCCACCGTGCA |
| FeoB.V5.Rev | CGGTGGAGTCCAAGCCAAGCAAGGGGGTTGGGGATGGGCTTGCCATGCA |
| FeoC.V5.Fwd | GATCCGGCAAGCCCAYCCCCAACCCCYTGCTTGGCTTGGACTCCACCTGAT |
| FeoC.V5.Rev | CATGATCAGGTGGAGTCCAACCCAAGCAAGGGGTTGGGGATGGGCTTGCCG |
| pFeoAB-V5C.FeoB.K15D.Fwd | GACCATTGAATAAGGTTGTGTCTCCACTATTCGGGTTGCCTACG |
| pFeoAB-V5C.FeoB.K15D.Rev | CGTAGGCAACCCGAATAGTGGAGACACAACCTTATTCAATGGTC |
| pFeoAB-V5C.FeoB.T36K.Fwd | TACCCGTTTTTTTCTCAACTTTGACCCCCGCCCAGTTAC |
| pFeoAB-V5C.FeoB.T36K.Rev | GTAACTGGGCGGGGGTCAAAGTTGAGAAAAAAACGGGTA |
| pFeoAB-V5C.FeoB.D55K.Fwd | AAAGCATAAATTCCGGGCAGCTTGGTGAGTGAAAATTCATCGC |
| pFeoAB-V5C.FeoB.D55K.Rev | GCGATGAATTTTCACTCACCAAGCTGCCCGGAATTTATGCTTT |
| pFeoAB-V5C.FeoB.D72A.Fwd | TAATGACAGTAACAGTATTGCGGAATCGATCGCATCGCGTGC |
| pFeoAB-V5C.FeoB.D72A.Rev | GCACGCGATGCGATCGATTCCGCAATACTGTTACTGTCATTA |
| pFeoAB-V5C.FeoB.N119K.Fwd | CGCTTAAGCGCATCCATTTTCTTAAGTACGACAATCATTGG |
| pFeoAB-V5C.FeoB.N119K.Rev | CCAATGATTGTCGTACTTAAGAAAATGGATGCGCTTAAGCG |
| pFeoAB-V5C.FeoB.D122N.Fwd | TCGCGCTTAAGCGCATTCATTTTGTTAAGTACGACAATCA |
| pFeoAB-V5C.FeoB.D122N.Rev | TGATTGTCGTACTTAACAAAATGAATGCGCTTAAGCGCGA |
| pFeoAB-V5C.FeoA.G32K.Fwd | CGACTTCGGTATTGGGGAGAACTTTCATCACCATCAGTTTCTTTCTG |
| pFeoAB-V5C.FeoA.G32K.Rev | CAGAAAGAAACTGATGGTGATGAAAGTTCTCCCCAATACCGAAGTCG |
| pFeoAB-V5C.FeoA.A45D.Fwd | CGTATTAATTCGCCGAGATCCAATGGGTGATCCGC |
| pFeoAB-V5C.FeoA.A45D.Rev | GCGGATCACCCATTCGAGCTCGGCGAATT |
| pFeoAB-V5C.FeoA.P50R.Fwd | GTACTTCAACTTGAAGCCGATCACCCATTGGAGCT |
| pFeoAB-V5C.FeoA.P50R.Rev | AGCTCCAATGGGTGATCGGCTTCAAGTTGAAGTAC |
| pFeoAB-V5C.FeoA.V72K.Fwd | CATCATTGTGCTCTCTCTCCTTATCAATATTTGCCGCAATATTTTCGC |
| pFeoAB-V5C.FeoA.V72K.Rev | GCGAAAATATTGCGGCAAATATTGATAAGGAGAGAGCACAATGATG |