TABLE 4.

Effect of FeoB mutations on growth of EPV6 and on Feo complex formation

Protein or mutation(s)a	Mutation location	Feo functionb	Feo complex formation
WT FeoB-V5	NAc	+	+
FeoB K15D	FeoB G1	−	−
FeoB T36K	FeoB switch I/G2	−	+
FeoB D55K	FeoB G3	−	+
FeoB T36K and D55K	FeoB switch I/G2/G3	−	+
FeoB D72A	FeoB switch II	−	−
FeoB N119K	FeoB G4	+	NDe
FeoB D122N	FeoB G4	+	ND
FeoB D93K	FeoB potential G4	+	ND
ΔCd	NA	−	+
FeoA G32K	FeoA	−	−
FeoA A45D	FeoA	−	−
FeoA P50R	FeoA	−	−
FeoA V72K	FeoA	−	−

^aAll the indicated mutations were made in the plasmid pFeoAB^-V5C.

^bFeo function was assessed by comparing growth of EPV6 carrying a mutated pFeoAB^-V5C plasmid to that of EPV6/pFeoAB^-V5C on LB agar without heme.

^cNA, not applicable.

^dΔC indicates the pFeoAB^-V5 plasmid.

^eND, not determined.