FIG 3.

Degradation of ethylene glycol by resting cells of A. woodii goes along with the formation of acetate and ethanol. Resting cells (1 mg/ml) of A. woodii were incubated in 10 ml imidazole buffer (50 mM imidazole, 20 mM KCl, 50 mM MgSO₄, 2 mM DTE, 4 μM resazurin, pH 7.0) containing 20 mM ethylene glycol in a 100% N₂ atmosphere at 30°C. The concentrations of ethylene glycol (▼), acetate (▲), and ethanol (■) were determined by gas chromatography. Acetaldehyde could not be detected under the conditions tested. All data points are means ± SEMs (n = 3 independent experiments).