FIG 6.

Characterization of the NADH:acetaldehyde oxidoreductase activity in extract of A. woodii cells grown on ethylene glycol. (A) Electron transfer from NADH to acetaldehyde in cell extracts was measured in a 100% N₂ atmosphere at 30°C in buffer containing 0.1 M Na₄P₂O₇, 20 mM MgSO₄, 22 mM glycine, 2 mM DTE, pH 9.0. NADH and acetaldehyde were added to concentrations of 0.5 and 10 mM, respectively. Michaelis-Menten kinetics for NADH (B) and acetaldehyde (C) are shown. All data points are means ± SEMs (n = 3 independent experiments).