FIG 7.
Model of ethylene glycol metabolism in A. woodii. For explanations, see the text. (A) Initial disproportionation of ethylene glycol to acetate and ethanol; (B) the following oxidation of ethanol to acetate (27). Note that the WLP requires H2, Fd2−, and NADH as reductant. Reduction of ferredoxin with NADH as reductant catalyzed by the Rnf complex requires reverse electron transfer, which is driven by the electrochemical sodium potential that is established by ATP hydrolysis (27). Here, 0.45 mol of ATP has to be hydrolyzed to drive the reaction.