FIG 4.

Role of the cidABC genes on ROS production and survival. (A) S. aureus ΔsrrAB, ΔsrrAB ΔcidA, ΔsrrAB ΔcidB, and ΔsrrAB ΔcidC mutants were collected at 1 and 3 days of growth in TSB plus 35 mM glucose at 37°C under aerobic conditions, stained with CTC and HPF, and then analyzed by flow cytometry. (B) Cell viabilities (mean ± SEM) were monitored every 24 h over a period of 5 days in TSB plus 35 mM glucose. (C) Acetate concentrations in culture supernatants were measured every 24 h over a period of 5 days using a commercially available kit (R-Biopharm). (D) pHs of the culture supernatants. (E) Growth of the S. aureus mutants in the presence of 5 mM H₂O₂ was monitored for 24 h at 37°C in a Tecan Infinite 200 spectrophotometer under maximum aeration.