TABLE 1.
Strains used in this study
| Strain | Relevant characteristic(s) | Reference or source |
|---|---|---|
| S. aureus | ||
| UAMS-1 | Clinical osteomyelitis isolate, rsbU+ | 34 |
| RN4220 | Highly transformable strain; restriction deficient | 35 |
| KB1058 | UAMS-1 ΔcidC | 13 |
| KB1060 | UAMS-1 ΔcidB, markerless | This study |
| KB1065 | UAMS-1 ΔcidAΔ2–52, markerless | This study |
| KB6004 | UAMS-1 ΔsrrAB, markerless | 20 |
| KB6005 | UAMS-1 ΔsrrAB ΔcidABC, markerless | This study |
| KB6006 | UAMS-1 ΔsrrAB ΔcidA, markerless | This study |
| KB6007 | UAMS-1 ΔsrrAB ΔcidB, markerless | This study |
| KB6008 | UAMS-1 ΔsrrAB ΔcidC, markerless | This study |
| E. coli | ||
| BL21 | Expression strain, F– ompT hsdS (rB− mB−) gal dcm (DE3) | Novagen |
| DH5a | Host strain for construction of recombinant plasmids | 36 |