TABLE 2.
Plasmids used in this study
| Plasmid | Relevant characteristic(s)a | Reference or source |
|---|---|---|
| pAJ22 | β-Galactosidase reporter plasmid; Cmr | 25 |
| pCL52.2 | Temp-sensitive shuttle vector; Tcr Spr | 19 |
| pCM12 | E. coli/S. aureus shuttle vector with PsarA-sodARBS-gfp (superfolder); Ampr Spr | 24 |
| pCN51 | Pcad-inducible plasmid; Ampr Ermr | 22 |
| pET24b | IPTG-inducible E. coli expression plasmid | Novagen |
| pET24b-srrA | pET24b with gene encoding SrrA | This study |
| pIHW5 | Pcad-inducible lacZ reporter plasmid; Ampr Ermr | This study |
| pIHW7 | Promoterless lacZ reporter plasmid; Ampr Ermr | This study |
| pIHW10lac | cidABC reporter plasmid; Ampr Ermr | This study |
| pIHW58 | srrAB complementation plasmid; Tcr Spr | This study |
| pJB12 | Temp-sensitive UAMS-1 ΔcidB plasmid (pCL52.2) | This study |
| pJB60 | Temp-sensitive allelic-exchange plasmid with counterselection; Ampr Cmr | This study |
| pJB60-114115 | Temp-sensitive UAMS-1 ΔcidABC plasmid; Ampr Cmr | This study |
| pJB61 | Temp-sensitive allelic-exchange plasmid with counterselection; Ampr Cmr | This study |
| pJB67 | pCN51 with TIR; Ampr Ermr | This study |
| pJB94 | E. coli/S. aureus shuttle vector; Tcr Spr | This study |
| pJB97 | UAMS-1 cidB complement plasmid; Ampr Ermr | This study |
| pRN8298 | pI258 replicon; Ampr Ermr | 22 |
a
TIR, optimized transcription initiation region.