TABLE 3.
Primers used in this studya
| Primer | Sequence (5′ to 3′) |
|---|---|
| IW3 | gcccgggATGACCATGATTACGGATTCACTGGCCGTC |
| IW4 | gggcgcgccTTATTTTTGACACCAGACCAACTGGTAATGG |
| IW11 | ggctagCGCCATCCCTTTCTAAATATGTCTAAATTGTTAC |
| IW15 | ggatccCATATTAATAAAGCACTCATTATTTGTGATTCC |
| IW91c | gggaattcGAACAGCGTAGCCAACAATTAATTACTACTGA |
| IW92c | ggggatccACATGCTTTTCTTTACAAAAGTATTATATCAC |
| IW93c | ggggatccTAAAATTGAATATAGTTATTTCAGAACGCATG |
| IW94c | ggtctagaGTAATTGTCTTTAGTGCTAAATAAAGTTGTAA |
| IW100 | GACGCCTCATGAAGTAAAAGTGATGCGTCA |
| IW101 | ATAGTTGATATTCGCAAAAACCCTAAACCC |
| IW114 | ggctagcTGATTGAAAGGTTATCACAATTGAATTGAA |
| IW115 | ggggtcgacCCAGAACGGTGAATAGAAAATATGATGTAA |
| IW118cc | ggctagcGTGGGAGGTATGACCTGTATGTCGAACG |
| IW119ccc | gctcgagTTTAGCCGGCTCATCATTAGATTTAACCTCAAATTTATACC |
| IW138 | ggagctcACAATAACAGAAGGTCGTAATCGTCAAGTC |
| IW139 | gggatccTTATTCTGGTTTTGGTAGTTTAATAATAAA |
| IW147 | ggggatccGTGGGAGGTATGACCTGTATGTCGAACG |
| IW152 | ggggtcgacTTTAGCCGGCTCATCATTAGATTTAACCTCAAATTTATACC |
| IW154 | GCTTATGAACTTGCAATGGAG |
| IW155 | CAGTTGATACTCATGTTAAACGAC |
| IW156 | GGGCTCATCTCAAACATATATTTTG |
| IW157 | CGGAAATGCGTGATTTAGAAATG |
| IW168 | Biotin- ATAGTTATTGTAACAATTTAGACATATTTAGAAAGGGATGGCGCCATGCACAAAGTCCAA |
| IW169 | TTGGACTTTGTGCATGGCGCCATCCCTTTCTAAATATGTCTAAATTGTTACAATAACTAT |
| IW170 | ATAGTTATTGTAACAATTTAGACATATTTAGAAAGGGATGGCGCCATGCACAAAGTCCAA |
| JBGFP4 | CGAATTCTTATTTGTAGAGCTCATCCATGCCATGTG |
| JBGFP8 | CCATATGCCCGGGagcaaaggagaagaacttttcactgg |
| JBI258ORI1 | ccagatcTGGCGAATGGCGCCGTTTTATCTTCATCAC |
| JBI258ORI2 | ggtgtacaGGGCCCTCGATGATTACCAGAAGTTCTCAC |
| JBCACOMP1 | cggatCCGCATGCAAATTATCAATGATGAAGTAGATATAGGC |
| JBCDALAC3 | cgtcgacCCATGCTTGTAATGCTTTAACTAATGCTTC |
a
All primers were generated in this study. Lowercase letters indicate nonhomologous regions of DNA.