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Growth attributes of S. aureus glucosaminidase mutants. Growth curves of S. aureus Newman (WT) and single (A), double (B), and triple and quadruple glucosaminidase mutants (C) and the complemented single sagB mutant (D). Overnight cultures were normalized to an A600 of 3.0, diluted 1:50, and monitored by recording optical density over time.
