FIG 7.
Wall teichoic acids of S. aureus glucosaminidase mutants. Murein sacculi of S. aureus Newman and its variants were isolated from mid-exponential-growth cultures (A600 of 0.6). (A) Cell wall phosphates were acid hydrolyzed from murein sacculi and quantified by colorimetric assay. Statistical significance of phosphate levels from at least three independent murein sacculus isolations was determined by one-way ANOVA, followed by Bonferroni's multiple-comparison test to wild-type levels (***, P < 0.001). (B) Wall teichoic acid was released from murein sacculi by 0.1 M NaOH treatment, separated by PAGE, and visualized with alcian blue-silver staining.