TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmid	Descriptiona	Reference(s) or source
Strains
S. aureus Newman	Wild type, clinical isolate	42, 75
atl mutant	Δatl::Sp	This work
sagB mutant	ΔsagB::aphA	This work
sagA mutant	ΔsagA	This work
scaH mutant	scaH::erm	This work
atl sagB mutant	Δatl::Sp ΔsagB::aphA	This work
atl sagA mutant	Δatl::Sp ΔsagA	This work
atl scaH mutant	Δatl::Sp scaH::erm	This work
sagAB mutant	ΔsagB::aphA ΔsagA	This work
sagB scaH mutant	ΔsagB::aphA scaH::erm	This work
sagA scaH mutant	ΔsagA scaH::erm	This work
atl sagAB mutant	Δatl::Sp ΔsagB::aphA ΔsagA	This work
atl sagB scaH mutant	Δatl::Sp ΔsagB::aphA scaH::erm	This work
atl sagA scaH mutant	Δatl::Sp ΔsagA scaH::erm	This work
sagAB scaH mutant	ΔsagB::aphA ΔsagA scaH::erm	This work
atl sagAB scaH mutant	Δatl::Sp ΔsagB::aphA ΔsagA scaH::erm	This work
atl sagAB scaH lytP2 lytP4 mutant	atl::erm ΔsagAB ΔscaH lytP2::Cm lytP4::Sp	Lab collection
USA300 LAC	Wild type, clinical MRSA isolate	55
USA300 sagB	USA300 ΔsagB::aphA	This work
Plasmids
pΔatl::Sp	pKOR1 with Δatl::Sp for allelic replacement	This work
pΔsagB::aphA	pKOR1 with ΔsagB::aphA for allelic replacement	This work
pΔsagA	pKOR1 with ΔsagA for allelic replacement	This work
pOS1	E. coli/S. aureus shuttle vector	32
psagB	sagB ORF and 852 bp upstream cloned into pOS1	This work
pET15b-AtlGL	atl3103–3771 cloned into the BamHI site of pET-15bb	This work
pET15b-sagB91–855	sagB91–855 cloned into the NdeI and BamHI sites of pET-15b	This work
pGEX-AtlAM	gst-atlAM fusionc	76
pGEX-AtlGL	gst-atlGL fusiond	76

^aORF, open reading frame.

^batl_3103–3771, atl gene encoding residues 3103 to 3771 of Atl.

^catl_AM, the atl gene encoding the Atl N-acetylmuramoyl-l-Ala-amidase.

^datl_GL, the atl gene encoding Atl N-acetylglucosaminidase.