TABLE 2.
Mass spectrometry identification of proteins with diminished secretion in sagB mutant S. aureus
| Slice | Protein | Localizationa | Mass (kDa) | Total no. of peptides | % coverage | Intensityb |
|---|---|---|---|---|---|---|
| 1 | vWbp | S | 59.24 | 541 | 77.95 | 11.6:1 |
| Coa | S | 71.67 | 324 | 75 | 6:1 | |
| NWMN_0401 | S | 56.51 | 288 | 86.5 | 16:1 | |
| 2 | PrsA | TM | 35.62 | 170 | 75.94 | 1:6.6 |
| PhdB | C | 35.22 | 86 | 77.3 | 1:7.4 | |
| GapA | C | 36.25 | 44 | 62.8 | 1:7.8 | |
| 3 | Ssl11 | S | 25.35 | 271 | 86.22 | 8.7:1 |
| Ssl7nm | S | 26.15 | 194 | 74.46 | 104:1 | |
| Ssl1nm | S | 25.63 | 125 | 72.12 | 17:1 | |
| 4 | NWMN_2203 | S | 17.39 | 101 | 54.22 | 1:7.3 |
| NWMN_0364 | C | 21.29 | 59 | 62.63 | 1:14.5 | |
| 5 | Fib | TM | 18.75 | 345 | 78.18 | 5.5:1 |
| Chp | S | 17.05 | 271 | 81.88 | 6.7:1 | |
| 6 | Scn | S | 13.06 | 213 | 61.21 | 1.25:1 |
| NWMN_1066 | S | 12.59 | 165 | 65.14 | 2.2:1 |
a
Cellular localization was deduced by analysis of amino acid sequences using SignalP, version 4.1, and TMHMM, version 2.0, servers. S, secreted; TM, transmembrane; C, cytoplasmic.
b
Data represent the ratio of values for the wild type to those for the sagB strain.