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. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: Nat Protoc. 2015 Jul 23;10(8):1264–1274. doi: 10.1038/nprot.2015.080

Figure 2.

Figure 2

Microscopic appearance of hepatocellular carcinoma in mice with cirrhotic liver. (a) Immunofluorescence staining of collagen I in mice with liver cirrhosis (Laennec 4 score) after 12 weeks of CCl4 treatment. In blue, DAPI cell nuclear counterstaining. (b,c) Masson’s trichrome staining of liver tissue from C3H (b) or Stk-mutant (c) mice with cirrhosis. (d,e) Representative images of CD8+ T lymphocyte infiltration (green, anti-CD8 antibody) in HCA-1 (d) and Stk-mutant tumors (e) with DAPI (VECTASHIELD) for nuclear counterstaining, in blue. Institutional regulatory board permission from MGH IACUC was obtained for all procedures performed within this protocol.